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Hi, I'm new to RNA-Seq and am looking for some information on the quality/size profile of RNA needed to produce high-quality data on either the 454 or Illumina platform. We are working with some environmental samples, and the goal is to sequence total prokaryotic community RNA. A MoBio soil RNA extraction kit produced decent results from an E.coli test culture but a freshly collected environmental sample looks rather degraded to my eye (see attached Bioanalyzer trace from the RNA Pico kit). I haven't found anything information on how to connect Bioanalyzer trace quality with library outcomes. Can anyone help?
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist on Modified Bases...Today, 07:01 AM -
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM
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