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Thread | Thread Starter | Forum | Replies | Last Post |
Threshold quality score to determine the quality read of ILLUMINA reads problem | edge | Illumina/Solexa | 35 | 11-02-2015 11:31 AM |
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BWA mapping quality score is unstable | mschatz | Bioinformatics | 0 | 09-15-2011 12:03 PM |
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What cut off does GATK snp calling use for mapping quality score? | foxyg | Bioinformatics | 0 | 09-25-2010 12:19 PM |
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#1 |
Member
Location: Ospedali Riuniti di Bergamo, ITALY Join Date: Oct 2009
Posts: 99
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Hi NGS users,
someone could explain me the different between MAPPING QUALITY and PER-BASE QUALITY SCORE? The score for the pileup is the Phred score of mapping quality? I have a bit of confusion about it! Thanx a lot ME |
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#2 |
Member
Location: Ospedali Riuniti di Bergamo, ITALY Join Date: Oct 2009
Posts: 99
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The mapping quality can be extracted from SAM or BAM file. But is there a way to do this using BIO:
![]() To extract mapping quality using Bio: ![]() $quality = $align->qual ? It returns the quality score for the alignment as a whole. Last edited by m_elena_bioinfo; 09-02-2010 at 06:53 AM. |
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#3 |
Nils Homer
Location: Boston, MA, USA Join Date: Nov 2008
Posts: 1,285
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Read the supplementary materials in the Li et al. MAQ paper (Genome Research).
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