Hello
First I want to apologize if there's a similar question already, but I can't seem to find an answer here.
I have to fastq files, one containing forward, one reverse mate pair and they are in the same order in both files. Each of those pairs overlap and what I want to do is to somehow merge them into one sequence to get a consensus sequence based on their qualities. These are Sanger sequences and are a bit longer.
Is there any software or a script that I could use for that? So far I've tried CAP3 and didn't manage to get desired results.
Thanks in advance.
First I want to apologize if there's a similar question already, but I can't seem to find an answer here.
I have to fastq files, one containing forward, one reverse mate pair and they are in the same order in both files. Each of those pairs overlap and what I want to do is to somehow merge them into one sequence to get a consensus sequence based on their qualities. These are Sanger sequences and are a bit longer.
Is there any software or a script that I could use for that? So far I've tried CAP3 and didn't manage to get desired results.
Thanks in advance.
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