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  • Bowtie error - FASTQ reads are too long

    Hi,

    I'm just aligning a MiSeq fastq run to a custom index and am getting the following error:

    FASTQ read name is too long; read names must be 1022 characters or fewer.
    Beginning of bad read name:
    terminate called after throwing an instance of 'int'
    /local/scratch/1426970873.9330346: line 8: 25641 Aborted

    I cant find anything online and am not sure how to fix this. Any thoughts?

  • #2
    It's likely that your fastq file is corrupt. I've never heard of a read having a name that would be longer than ~50 characters, let alone 1022.

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