SEQanswers

Go Back   SEQanswers > Sequencing Technologies/Companies > Illumina/Solexa



Similar Threads
Thread Thread Starter Forum Replies Last Post
How do i get rid of Formamide from my dsDNA? mzahir89 Sample Prep / Library Generation 5 10-08-2015 04:05 AM
QuantiFluor dsDNA Dye System GSviral 454 Pyrosequencing 0 12-17-2014 06:50 AM
Circle graphs JohnK Bioinformatics 2 01-28-2011 06:50 AM
PubMed: Massively parallel display of genomic DNA fragments by rolling-circle amplifi Newsbot! Literature Watch 0 07-07-2010 11:20 AM

Reply
 
Thread Tools
Old 11-20-2018, 08:32 AM   #1
hoytpr
Member
 
Location: Stillwater

Join Date: Dec 2009
Posts: 53
Default Rolling circle dsDNA vs ssDNA experience?

Does anyone have experience with samples using rolling circle amplification that can tell me whether they have relatively high or low amounts of ssDNA? The client is not local so it would help to know in advance whether they need to ship much more based on A260 readings before starting library preps.
Thanks,
-p
hoytpr is offline   Reply With Quote
Old Yesterday, 11:41 AM   #2
GW_OK
Senior Member
 
Location: Oklahoma

Join Date: Sep 2009
Posts: 405
Default

Comparing A260 vs picogreen/Qubit should tell you if there's a large amount of ssDNA present.
GW_OK is offline   Reply With Quote
Reply

Tags
rca, rolling circle

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 12:20 PM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2018, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO