Hi,
the title says it all.
I am doing Trizol extractions of total RNA for subsequent RNAseq library preps from small tissue samples. I'm wondering if DNAse treatment and/or LiCl precipitation are necessary to remove residual gDNA from my total RNA extracts.
Once again I'm referring to the Sengupta et al paper (in which they describe the preparation of RNAseq libraries from 10 ng total RNA) as their initial RNA purification is remarkably simple.
the title says it all.
I am doing Trizol extractions of total RNA for subsequent RNAseq library preps from small tissue samples. I'm wondering if DNAse treatment and/or LiCl precipitation are necessary to remove residual gDNA from my total RNA extracts.
Once again I'm referring to the Sengupta et al paper (in which they describe the preparation of RNAseq libraries from 10 ng total RNA) as their initial RNA purification is remarkably simple.
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