Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
Collapse
 
  • Page of 1
  • Filter
  • Time
  • Show
Clear All
new posts
Previous template Next
  • #1

    TruSeq RNA master mix substitution

    Our lab has been making RNA libraries using a TruSeq RNA kit v2. Right now we have enough of all of the kit reagents for our libraries, except for the second strand master mix.

    Has anyone tried substituting other second strand enzyme mix in this protocol? Or using a different protocol from the one in the kit to convert RNA to cDNA before proceeding with library production?
    Tags: illumina, protocols, rna, truseq
  • #2
    Hi, my experience with the protocol is that is not easy to get it right even following the protocol and using what they suggest..
    BW

    Comment

    Previous template Next

    Latest Articles

    Collapse
    • seqadmin
      Recent Advances in Sequencing Analysis Tools
      by seqadmin


      The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...
      Today, 07:48 AM
    • seqadmin
      Essential Discoveries and Tools in Epitranscriptomics
      by seqadmin




      The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...
      04-22-2024, 07:01 AM
    View All

    ad_right_rmr

    Collapse

    News

    Collapse
    Topics Statistics Last Post
    Enhanced Neoantigen Detection: Introducing NeoHunter by seqadmin
    Started by seqadmin, Today, 07:17 AM
    0 responses
    11 views
    0 likes
    		 Last Post seqadmin
    by seqadmin
    Today, 07:17 AM  
    A Close Examination at Probiotic-Related Bacteremia by seqadmin
    Started by seqadmin, 05-02-2024, 08:06 AM
    0 responses
    19 views
    0 likes
    		 Last Post seqadmin
    by seqadmin
    05-02-2024, 08:06 AM  
    Expanded Genetic Insights into Blood Pressure Regulation by seqadmin
    Started by seqadmin, 04-30-2024, 12:17 PM
    0 responses
    20 views
    0 likes
    		 Last Post seqadmin
    by seqadmin
    04-30-2024, 12:17 PM  
    The Role of Enhancers in Defining Cell Fate by seqadmin
    Started by seqadmin, 04-29-2024, 10:49 AM
    0 responses
    28 views
    0 likes
    		 Last Post seqadmin
    by seqadmin
    04-29-2024, 10:49 AM  
    View All
    Working...
    X