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Old 01-13-2014, 02:16 PM   #1
GW_OK
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Default New thing?

Now is the time of day for WILD SPECULATION!

Alright, so:
1) JP Morgan Healthcare conference. Prime announcement venue as shown previously.
2) Eco and Pathogenomenick are twittering they know something we don't know.
3) Somebody told me Illumina is gearing up for a big marketing blitz "very soon".
4) A source I'm not going to disclose asked me if I owned or was going to purchase an "Illumina Hiseq 1T".
5) I've heard or read rumors over the past year that Illumina might put out a "midi-seq", smaller than a Hiseq, bigger than a Miseq.

Anybody care to give odds over something big being announced soon-ish?

Edit: I have just been informed that the Hiseq 1T is going to be the name for the upgraded Hiseqs which can do those 6-day 1Tbp runs. The upgrade you most likely do not qualify for.

Last edited by GW_OK; 01-13-2014 at 02:48 PM.
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Old 01-13-2014, 02:49 PM   #2
Richard Finney
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This thing ?

http://www.genomeweb.com/sequencing/...ushed-mid-2014

Illumina to Launch 1 Terabase HiSeq Upgrade in Q2, 2014; 2x250 bp Reads Pushed to Mid-2014
October 29, 2013
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Old 01-13-2014, 05:34 PM   #3
bilyl
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Quote:
Originally Posted by GW_OK View Post
Now is the time of day for WILD SPECULATION!

Alright, so:
1) JP Morgan Healthcare conference. Prime announcement venue as shown previously.
2) Eco and Pathogenomenick are twittering they know something we don't know.
3) Somebody told me Illumina is gearing up for a big marketing blitz "very soon".
4) A source I'm not going to disclose asked me if I owned or was going to purchase an "Illumina Hiseq 1T".
5) I've heard or read rumors over the past year that Illumina might put out a "midi-seq", smaller than a Hiseq, bigger than a Miseq.

Anybody care to give odds over something big being announced soon-ish?

Edit: I have just been informed that the Hiseq 1T is going to be the name for the upgraded Hiseqs which can do those 6-day 1Tbp runs. The upgrade you most likely do not qualify for.
The "midi-seq" is effectively the Rapid Run kits on the Hiseq. It has practically the same number of reads as the old GA systems (hundreds of millions). The high output mode is in the billions of reads, and Miseq is in the 10s of millions, so there's not much more wiggle room there.

My guess is that the rapid run kits will be going 2x250bp real soon or earlier than expected. There's a big hole in the Miseq v3 kits (no 2x150 cycle kits) so maybe that will be filled up as well.

Honestly, Illumina is doing so well right now it's hard to see where they could push further besides increasing sequencing capacity. The Hiseq software could use a nice upgrade in terms of applying the newer Miseq RTA that handles low diversity libraries very well, but that's probably low on their priority list.
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Old 01-14-2014, 07:07 AM   #4
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What they could really use is a middle instrument. That's what I mean by "midi-seq" previously.

Imagine something that could only run a single, Rapid-only flowcell. Priced around twice what a Miseq runs, which would still be less than half of what a Hiseq costs. You could claim the 1500's are doing this now, but they're like 80% of what a 2500 costs, so why not pay the full price and get the big guy? I think there's still labs out there who would spring for something bigger than a Miseq but can't justify the cost of a 2500.
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Old 01-14-2014, 07:17 AM   #5
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Quote:
The Hiseq software could use a nice upgrade in terms of applying the newer Miseq RTA that handles low diversity libraries very well, but that's probably low on their priority list.
It is not. I was told it would be released this year.
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Old 01-14-2014, 08:44 AM   #6
GW_OK
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Called it! Called it!

Holy sh_!

"NextSeq"
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Old 01-14-2014, 09:30 AM   #7
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NextSeq 500: http://www.illumina.com/systems/nextseq-sequencer.ilmn

Last edited by GenoMax; 01-14-2014 at 10:16 AM.
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Old 01-14-2014, 09:57 AM   #8
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Don't forget the 10-pack of uber HiSeqX machines:

http://nextgenseek.com/2014/01/illum...0-at-jpm-2014/
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Old 01-14-2014, 10:03 AM   #9
GenoMax
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Now that is exciting.

HiSeq X Ten page at Illumina: http://illumina.com/systems/hiseq-x-...ng-system.ilmn

HiSeq X10 - $10M for 10 machines per Eli Roberson.

Quote:
The HiSeq X Ten is sold as a set of 10 or more ultra-high throughput sequencing systems, each generating up to 1.8 terabases (Tb) of sequencing data in less than three days or up to 600 gigabases (Gb) per day, per system

Last edited by GenoMax; 01-14-2014 at 10:21 AM.
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Old 01-14-2014, 11:15 AM   #10
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I calculated you could do ~20,800 30x human genomes if you ran all 10 twice a week for 1 year.
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Old 01-14-2014, 11:16 AM   #11
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Really, this is a bit of a giant FU to Complete Genomics and, uh, BGI.
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Old 01-14-2014, 11:41 AM   #12
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The rep at PAG said they planned on selling 4 "X boxes" in 2014--and had already sold 3 30 minutes after the announcement.

The NextSeq500 is clearly aimed at people considering a Proton. Same price, same emphasis on speed, better stats right now. I don't like that the only single-end mode is 75 bp. Lots of people like the 150 bp read on the 2500 Rapid. And the other downside is that all 4 lanes (100M reads each) get fed the same library, so doesn't improve flexibility in flow of projects at a facility. But a nice mid-machine.
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Old 01-14-2014, 12:16 PM   #13
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What I'd like is some more detailed explanation of the 2-dye system the NextSeq 500 is using. I assume they provide C & A tagged with Red, T & A tagged with green & G untagged, thus C should be pure Red, T pure green and A ~ a 50:50 mix of green & red (orange) and G then undyed...
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Old 01-14-2014, 01:23 PM   #14
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Interested to see that the cluster density specs are an order of magnitude lower on the 500 than the MiSeq. Is that an error?

Quote from Illumina:
(http://www.illumina.com/systems/next...fications.ilmn)
* Install specifications based on Illumina PhiX control library at supported cluster densities (between 129 and 165 k/mm˛ clusters passing filter). Actual performance parameters may vary based on sample type, sample quality, and clusters passing filter.
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Old 01-14-2014, 01:58 PM   #15
Richard Finney
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Illumina announces $1,000 genome

??

http://www.utsandiego.com/news/2014/...dollar-genome/

"The cost of sequencing a human genome has at last been brought below $1,000, DNA sequencing giant Illumina said Tuesday. Crossing that barrier opens to door to bringing the public the full benefit of 21st-century genomic medicine."

via hacker news ... https://news.ycombinator.com/item?id=7060021
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Old 01-14-2014, 02:27 PM   #16
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Quote:
Originally Posted by GW_OK View Post
Called it! Called it!

Holy sh_!

"NextSeq"
Looks like I'll have to eat my hat! Exciting news!
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Old 01-14-2014, 02:51 PM   #17
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Quote:
Originally Posted by aeonsim View Post
What I'd like is some more detailed explanation of the 2-dye system the NextSeq 500 is using. I assume they provide C & A tagged with Red, T & A tagged with green & G untagged, thus C should be pure Red, T pure green and A ~ a 50:50 mix of green & red (orange) and G then undyed...
Spec Sheet from ILMN:
http://bit.ly/1iOYN7e
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Old 01-14-2014, 06:12 PM   #18
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New patent on the exclusion amplification for the X. http://bit.ly/1j6sSll

Just transport the dna fragments slowly and amplify quickly.
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Old 01-14-2014, 08:15 PM   #19
AllSeq
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Quote:
Originally Posted by GW_OK View Post
Called it! Called it!

Holy sh_!

"NextSeq"
I think you mean "Nailed it!" - really good call!
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Old 01-14-2014, 10:07 PM   #20
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Quote:
Originally Posted by M4TTN View Post
Interested to see that the cluster density specs are an order of magnitude lower on the 500 than the MiSeq. Is that an error?

Quote from Illumina:
(http://www.illumina.com/systems/next...fications.ilmn)
* Install specifications based on Illumina PhiX control library at supported cluster densities (between 129 and 165 k/mm˛ clusters passing filter). Actual performance parameters may vary based on sample type, sample quality, and clusters passing filter.
See flowcell pics on Twitter @nextgenseek
Fat lanes = different optics
Probably not a typo
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