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Old 01-20-2015, 06:34 AM   #1
jenyl
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Post Tools for identifying/verifying protein (without accession number)

If I have a series of protein sequences (experimentally obtained) that yield no results from BLAST, are there any other online/software tools that can be used to either identify or verify that these proteins exist?
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Old 01-20-2015, 06:39 AM   #2
GenoMax
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If you obtained these sequences experimentally then they definitely exist

Have you tried psi-blast or better yet delta-blast @NCBI?
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Old 01-20-2015, 06:46 AM   #3
jenyl
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They're not annotated in databases such as UniProt/Swiss-Prot so I'm not sure how I can "prove" that they are indeed real and transcribed from genes.

All I have are the protein sequences themselves though, won't I need accession numbers or ID to run them through psi/delta-blast?
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Old 01-20-2015, 07:22 AM   #4
GenoMax
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You just need fasta sequence for doing delta- or psi-blast. You can limit your search against swissprot or refseq if you want well annotated proteins.
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