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Old 09-08-2016, 01:58 PM   #1
spore
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Default Anyone have experience with the new Sequel chemistry and software?

PacBio announced an update to the Sequel software and chemistry about a month ago. Have any of the Sequel users here had any experience with it? Does it solve the read length and loading issues? Or is it still more practical to go with a RSII for sequencing.

Thank you
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Old 09-08-2016, 05:48 PM   #2
Brian Bushnell
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I just attended the PacBio conference today. It was fun! Lots of interesting presentations (mainly about how PacBio achieves assemblies with L50's 1000x longer than Illumina). And I learned something that I was not aware of before - the Sequel platform has not actually been released yet. It's still in development.

Therefore - at present, yes, it is more practical to go with RSII for sequencing.
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Old 09-09-2016, 11:41 AM   #3
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I thought the new update finally released the platform for production use? Or is this still not the case? Will we have to wait until the end of year update until service providers start services?

Has anyone tested the performance of the new chemistry yet?
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Old 09-19-2016, 12:03 PM   #4
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Quote:
Originally Posted by Brian Bushnell View Post
- the Sequel platform has not actually been released yet. It's still in development.
Im the product manager for the Sequel system and gave the morning talk at UGM highlighting our latest results on the system. Apologies if I mislead you on the status of our system. The Sequel system has been released and started shipping end of last year, see our Q1 financial Press Release
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Old 09-19-2016, 01:43 PM   #5
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I cannot remember any tweet on your talk and wonder if it's recording is available on line for viewing.
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Old 09-19-2016, 02:10 PM   #6
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Its not recorded as we have not been recording talks at our user group meetings.
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Old 09-20-2016, 04:38 AM   #7
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Im the product manager for the Sequel system.
So the burning question on everyone's mind. When do we get to see real data from Sequel in wild?
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Old 09-27-2016, 03:50 PM   #8
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So the burning question on everyone's mind. When do we get to see real data from Sequel in wild?
Check out today's blog: http://http://www.pacb.com/blog/sequ...nome-assembly/
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Old 09-27-2016, 05:50 PM   #9
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Is this using the August chemistry update or is it using the planned EOY chemistry update?
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Old 09-27-2016, 07:24 PM   #10
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It is currently in early access testing and released broadly thereafter, before the year end chemistry.
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Old 09-28-2016, 01:28 AM   #11
nucacidhunter
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If I am correct the Sequel data in the blog are not from current chemistry.
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Old 09-28-2016, 03:44 AM   #12
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If I am correct the Sequel data in the blog are not from current chemistry.
That data will be available next year
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Old 09-28-2016, 03:46 AM   #13
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Thanks. Does this mean the NDA (if there was one in place) is now lifted and others can start releasing their data?
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Old 10-24-2016, 12:05 PM   #14
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There has never been an NDA for Sequel or its data. Also the updated chemistry is available for order and will start shipping to customers this week.
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Old 10-24-2016, 12:28 PM   #15
nucacidhunter
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I wonder if this chemistry release is the last one for this year or there will be another chemistry or SMRT cell update before next year.
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Old 10-31-2016, 06:18 AM   #16
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seems like there is a surprising amount of misinformation. Thanks for clarifying.
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Old 11-10-2016, 02:15 AM   #17
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Hi all, has anyone tested the performance of the novel chemistry yet? Any experiences regarding loading efficiency, readoutput, dataquality and so on? I would really, appreciate any kind of information from all you sequel users out there!

Thanks!
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Old 11-11-2016, 10:51 AM   #18
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Hi All,
we have only run E.coli libraries with the new chemistry so far.
http://dnatech.genomecenter.ucdavis....pacbio-sequel/

Last edited by DNATECH; 11-11-2016 at 11:15 AM.
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Old 11-16-2016, 12:06 AM   #19
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Have a look at https://downloads.pacbcloud.com/publ...opsisDemoData/
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Old 11-17-2016, 11:22 PM   #20
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Hi all and thanks for your suggestions! I already recognised pacBios Arabidopsis release. However, the Sequel-data comes in a "new" format (*.bam) and one needs to use smrt-link to visualise the data. Samtools seems not to be feasible to have a look into the binary files. Is anyone aware of a workaround to open or even convert the files? Maybe to make them suitable for import into smrt-portal?
@DNATECH: Very helpful info!
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