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Old 06-21-2011, 05:05 AM   #1
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Location: Michigan

Join Date: Dec 2010
Posts: 5
Default Miltenyi Biotec cDNA synthesis kit

Hello all,

I'm just starting my thesis work and have been looking for ways preparing RNA-seq libraries (specifically for Illumina). As I work on yeast I have the option to multiplex quite heavily and have reached a point where library preps will cost more than the sequencing itself. Thus, I've been looking for cheap and high throughput ways to get to decent libraries.

I ran across Miltenyi Biotec's cDNA synthesis kit and it looks like it would be a great starting point, but haven't found a lot of information on the quality of the cDNA produced. I know their process is first strand synthesis only, but that is easy enough to fix/not a problem for some library prep procedures. I was wondering if anyone had experience with this kit and if so, how did they go about preparing libraries afterwards. Also, does anyone know of a relatively cheap method for preparing libraries in bulk? I've managed to get down to ~$45 using NebNext kits ordered through my university, but it is a bit daunting to think of the number of gels I'll have to go through.

Thanks all,
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