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Old 03-31-2012, 02:07 AM   #1
R_user
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Location: Springfield

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Default EdgeR Normalization Question

I am looking to do TMM normalization in an experiment where you have to do pairwise comparisons between 20+ samples in replicates of three (A1, A2, A3 vs B1, B2, B3; A1, A2, A3 vs C1, C2, C3; B1, B2, B3 vs C1,C2, C3 and so on).
I was thinking that I should normalize all the samples together and then do pairwise comparisons.
I was wondering how I can get EdgeR to give me the TMM normalized values in a csv file.
Thanks!
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Old 05-07-2012, 10:14 PM   #2
Gordon Smyth
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Default

There's actually no such thing as TMM normalized values. Normalization in edgeR is something that affects the fitted models rather than the data values. As the edgeR User's Guide says:

"There are methods implemented in edgeR to normalize the counts for compositional bias in sequenced libraries and for diff erences between libraries in sequencing depth. These adjustments are off sets in the models used for testing DE and do not transform the counts in any way."
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