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Old 02-16-2010, 08:39 PM   #1
RCJK
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Default Paper: Fully automated process for barcoded libraries for 454

Check this out for automated 454 general library prep:

http://genomebiology.com/2010/11/2/R15

"A scalable, fully automated process for construction of sequence-ready barcoded libraries for 454"
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Old 02-17-2010, 07:41 AM   #2
nickloman
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Very cool. I wish we could just buy it off the shelf and have it working tomorrow!
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Old 02-19-2010, 09:14 AM   #3
dottomarco
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This is based on the old library protocol right? Now the new rapid library protocol solved a lot of issues.. but it wold be interesting to apply the 96 parallelization idea of the paper to the new rapid protocol
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Old 06-23-2010, 05:46 AM   #4
james hadfield
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Fluidigm have a 454 amplicon prep method that allows you to make 48 libraries of up to 480 amplicons, barcoded and ready to sequence. All from one PCR.
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Old 06-23-2010, 08:17 AM   #5
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Oh yes, this thing? http://www.fluidigm.com/pr/2010/2010...essRelease.htm

Looks really handy for some of our apps.
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