Hi
Does anyone have experiences in using the scripture tool to assembly transcripts from RNA-seq data?
My RNA-seq data is directional paired-end data. When using scripture to assembly transcripts, it seems that scripture is not able to make use of the directional information in the RNA-seq and assemblied some transcripts without specific-strand information. Does anyone has encountered the similar problem? Any solutions?
Many thanks!
Does anyone have experiences in using the scripture tool to assembly transcripts from RNA-seq data?
My RNA-seq data is directional paired-end data. When using scripture to assembly transcripts, it seems that scripture is not able to make use of the directional information in the RNA-seq and assemblied some transcripts without specific-strand information. Does anyone has encountered the similar problem? Any solutions?
Many thanks!