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Old 04-05-2016, 08:04 AM   #1
Squall
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Location: Ireland

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Default DNA concentration methods, SureSelect

Hi all,

Just wondering if anyone had info or thoughts on whether concentrating a sheared DNA library using precipitation methods or water depletion methods (such as butanol extraction) would negatively effect the chemistry of the library going into, for example, a SureSelect preparation protocol?

Thanks!
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Old 04-05-2016, 05:15 PM   #2
nucacidhunter
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It will not change the library chemistry. To avoid negative impact on hybridisation one should be careful about carry over of salts or alcohols from the process.
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Old 04-06-2016, 12:27 AM   #3
Squall
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Great, thanks hunter!
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