Balance sequencing runs ATAC

Libby Evans · 04-23-2018, 06:51 AM

I am having trouble balancing my sequencing runs for ATAC-seq. I am doing extensive kapa on the samples but often finding one out of a pool of 6 will be far too high (on one occasion taking 40% of the total reads). These samples often look quite low by kapa. Has anyone experienced this?

If you do size correction how do you do this? (On the tapestation or bioanalyser?) Also do you typically do a size selection after the indexing clean up? I haven't included that yet but could. Any advice very welcome! Thanks.

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04-23-2018, 06:51 AM	#1
Libby Evans Junior Member Location: Oxford Join Date: Apr 2018 Posts: 1	Balance sequencing runs ATAC I am having trouble balancing my sequencing runs for ATAC-seq. I am doing extensive kapa on the samples but often finding one out of a pool of 6 will be far too high (on one occasion taking 40% of the total reads). These samples often look quite low by kapa. Has anyone experienced this? If you do size correction how do you do this? (On the tapestation or bioanalyser?) Also do you typically do a size selection after the indexing clean up? I haven't included that yet but could. Any advice very welcome! Thanks.