|
|
|
|
|||||||
Similar Threads
|
||||
| Thread | Thread Starter | Forum | Replies | Last Post |
| webbased full length EST identification | cdlam | Bioinformatics | 0 | 01-08-2013 03:54 PM |
| full length cDNA normalization | CPCantalapiedra | RNA Sequencing | 0 | 11-16-2012 01:06 AM |
| How to obtain full length RNA transcript sequence | Annibal | RNA Sequencing | 9 | 03-14-2012 07:51 AM |
| about UCSC full length cDNA | zslee | Bioinformatics | 6 | 05-18-2010 04:11 AM |
| PubMed: Use of massive parallel pyrosequencing for near full-length characterization | Newsbot! | Literature Watch | 0 | 09-16-2009 03:00 AM |
 |
|
|
Thread Tools |
03-19-2013, 01:26 PM
|
#1 |
|
Junior Member
Location: Colombia Join Date: Jan 2012
Posts: 2
|
Full-length cDNA and NGS
Hello everyone,
Is there such a thing such as full-length cDNA libraries that can be sequenced through NGS? Thanks, Héctor |
|
|
|
03-19-2013, 01:54 PM
|
#2 |
|
Senior Member
Location: Boston,MA Join Date: Nov 2008
Posts: 122
|
If by full length you mean sequence each base-yes. Shear it to appropriate size and make a library. There will be enouch overlap to reconstruct the full length.
If by full length you mean in one continuous sequence-no. Even Sanger does not go that long. |
|
|
|
|
03-20-2013, 10:11 AM
|
#3 |
|
Junior Member
Location: california Join Date: Mar 2008
Posts: 3
|
Not sure if this answers your question but here is a publication and poster showing full length cDNA sequencing to look at splice isoforms without assembly:
http://www.pacificbiosciences.com/pd...g_PacBioRS.pdf http://www.plosone.org/article/info%...l.pone.0046679 |
|
|
|
|
| Tags |
| cdna, full-length, libraries, ngs |
| Thread Tools | |
|
|
