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Old 05-24-2011, 02:27 AM   #1
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Location: wageningen

Join Date: May 2011
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Default reverse run -due to machine failure- only 45bp

Hi all,

I have a rather unusual question for which I get conflicting answers up to know. Hope anyone can be of help.
We have made a RRLibrary of 100-200bp fragments for GA2 sequebcing. The forward run is 100bp and the reverse run -due to machine failure- only 45bp. How does this influence assembly compared to 100bp from both runs? Do we n
eed to adjust settings in a short read assembler?

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