SEQanswers

Go Back   SEQanswers > Literature Watch



Similar Threads
Thread Thread Starter Forum Replies Last Post
NEXTflex ChIP-Seq Kit for Improved Genome Wide Analysis of DNA-Protein Interactions Bioo Scientific Vendor Forum 0 11-07-2011 09:25 AM
ChIP-Seq: An Integrated Pipeline for the Genome-Wide Analysis of Transcription Factor Newsbot! Literature Watch 0 03-02-2011 03:50 AM
ChIP-Seq: Genome-wide histone acetylation data improve prediction of mammalian transc Newsbot! Literature Watch 0 07-29-2010 03:30 AM
ChIP-Seq: Genome-wide Analysis using ChIP to Identify Isoform-specific Gene Targets. Newsbot! Literature Watch 0 07-21-2010 03:00 AM
PubMed: Genome-wide analysis of transcription factor binding sites based on ChIP-Seq Newsbot! Literature Watch 1 01-27-2009 05:26 AM

Reply
 
Thread Tools
Old 06-21-2011, 03:00 AM   #1
Newsbot!
RSS Posting Maniac
 

Join Date: Feb 2008
Posts: 1,443
Default ChIP-Seq: Genome-wide analysis of the relationships between DNaseI HS, histone modifi

Syndicated from PubMed RSS Feeds

Genome-wide analysis of the relationships between DNaseI HS, histone modifications and gene expression reveals distinct modes of chromatin domains.

Nucleic Acids Res. 2011 Jun 17;

Authors: Shu W, Chen H, Bo X, Wang S

To understand the molecular mechanisms that underlie global transcriptional regulation, it is essential to first identify all the transcriptional regulatory elements in the human genome. The advent of next-generation sequencing has provided a powerful platform for genome-wide analysis of different species and specific cell types; when combined with traditional techniques to identify regions of open chromatin [DNaseI hypersensitivity (DHS)] or specific binding locations of transcription factors [chromatin immunoprecipitation (ChIP)], and expression data from microarrays, we become uniquely poised to uncover the mysteries of the genome and its regulation. To this end, we have performed global meta-analysis of the relationship among data from DNaseI-seq, ChIP-seq and expression arrays, and found that specific correlations exist among regulatory elements and gene expression across different cell types. These correlations revealed four distinct modes of chromatin domain structure reflecting different functions: repressive, active, primed and bivalent. Furthermore, CCCTC-binding factor (CTCF) binding sites were identified based on these integrative data. Our findings uncovered a complex regulatory process involving by DNaseI HS sites and histone modifications, and suggest that these dynamic elements may be responsible for maintaining chromatin structure and integrity of the human genome. Our integrative approach provides an example by which data from diverse technology platforms may be integrated to provide more meaningful insights into global transcriptional regulation.

PMID: 21685456 [PubMed - as supplied by publisher]



More...
Newsbot! is offline   Reply With Quote
Reply

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 09:42 AM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2021, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO