Current read1, index, and read2 primers for HiSeq2000

SeqVicious · 09-26-2011, 02:49 PM

Hi all,

I'm currently being thrown into creating a library prep for some samples in our lab. We plan on multiplexing because and we want to throw in upwards of 6 samples. My lab has created library preps in the past but they used a different sequencing facility and older technology. We still have the left over reagents but I don't know if they will be compatible with HiSeq2000. Can someone tell me what the current read1, index, and read2 sequencing primers are? I have enough "barcodes" but I need to know what the sequence on either side of those "barcodes" are.

SeqVicious · 09-26-2011, 03:12 PM

Also, I've been trying to find these on my own but I guess I just don't know enough about this stuff yet. What are the SE and PE primers?

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09-26-2011, 02:49 PM	#1
SeqVicious Member Location: New Hampshire Join Date: Sep 2011 Posts: 20	Current read1, index, and read2 primers for HiSeq2000 Hi all, I'm currently being thrown into creating a library prep for some samples in our lab. We plan on multiplexing because and we want to throw in upwards of 6 samples. My lab has created library preps in the past but they used a different sequencing facility and older technology. We still have the left over reagents but I don't know if they will be compatible with HiSeq2000. Can someone tell me what the current read1, index, and read2 sequencing primers are? I have enough "barcodes" but I need to know what the sequence on either side of those "barcodes" are.

09-26-2011, 03:12 PM	#2
SeqVicious Member Location: New Hampshire Join Date: Sep 2011 Posts: 20	Also, I've been trying to find these on my own but I guess I just don't know enough about this stuff yet. What are the SE and PE primers?