Go Back   SEQanswers > Sequencing Technologies/Companies > Illumina/Solexa

Similar Threads
Thread Thread Starter Forum Replies Last Post
Does standalone Blat can take multiple chromosome in one command line, find matches? ketan_bnf Bioinformatics 2 06-07-2012 01:12 AM
Missing V600E mutation in WES of A375 cell line? Samtools problem? angerusso Bioinformatics 15 02-19-2012 06:02 PM
Request for sequencing a whole chromosome cell line Iredc27 General 2 10-05-2011 02:37 PM
Need Reference Annotation for MCF-7 Cell Line Transcripts AndrewCarpenter Bioinformatics 0 07-20-2010 07:52 AM
MCF-7 Cell Line Transcript Reference Annotation Needed AndrewCarpenter De novo discovery 0 07-19-2010 09:18 AM

Closed Thread
Thread Tools
Old 10-05-2011, 05:03 AM   #1
Junior Member
Location: Montevideo, Uruguay

Join Date: Oct 2011
Posts: 8
Default Request for sequencing a whole chromosome cell line

Hi to everyone, i need your help!
I am currently working in Cytogenetics in a lab located in Montevideo (Uruguay), and i am working with a mouse cell line. I am able to identify each chromosome of this cell line, based on its unique pattern of dark and light bands. I am interested in studying the sequence of the chromosome's pair 1. Through Chromosome microdissection I physically removes the complete chromosome of pair 1 from the slide. Then i procedure to make multiple copies of the isolated Chromosome using Sigma GenomePlex Single Cell Whole Amplification Kit, that work in three step 1)fragment the chromosome dna, 2)prepare a DNA library, 3)amplification of the DNA fragment
I need to sequence the whole chromosome, but in Montevideo, we don not account even with this new and incredible technology in sequencing DNA.
I was wonderng if this amplified sample of the entire chromosome (explaind before) would work if i send it to sequence with Illumina Hiseq2000 for example?

Which other things i have to take into consideration?

I really appreciate any response, criticisms or recommendations

Ire rolleyes:
Iredc27 is offline  
Old 10-06-2011, 02:49 AM   #2
Location: Oslo, Norway

Join Date: Nov 2008
Posts: 415

Closed due to cross-posting to in total four forums.
flxlex is offline  
Closed Thread

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 04:44 PM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2021, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO