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Thread | Thread Starter | Forum | Replies | Last Post |
about bwa aln parameter tuning questions | henry | Bioinformatics | 1 | 06-12-2012 08:31 AM |
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#1 |
Junior Member
Location: china Join Date: Apr 2011
Posts: 5
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Hi,
I aligned pair end reads using bwa aln -n 0.02 and 0.04. My expectation is there would be higher mapping rate using 0.04 than 0.02. However, I got more reads mapped to genome using 0.02 than 0.04. The other phenomenon is when I run bwa aln specifying -o 0, I got a output file contain reads those are gapped aligned. Can someone explain how these happened? Thank you. |
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