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Old 11-23-2013, 07:05 AM   #1
Palgrave
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Location: norway

Join Date: Aug 2011
Posts: 73
Default RIP-Seq

Hi,

I am doing RNA-IP on a RNA-binding protein and have the following question:

What is the best way to elute the protein with the RNA from the beads to preserve RNA quality?
I was thinking of using Glycine followed by Phenol extraction of the RNA.
The RNA is not crosslinked to the protein.
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