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08-02-2012, 04:46 PM
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#1 |
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Junior Member
Location: USA Join Date: Jul 2012
Posts: 1
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Kmer Content
Hi,
What can be the cause of an increased number of 5-mers AAAAA and TTTTT? Thank you. |
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08-10-2012, 05:11 PM
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#2 |
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Senior Member
Location: The University of Melbourne, AUSTRALIA Join Date: Apr 2008
Posts: 275
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If you are sequencing repetitive regions of Human DNA for example, you would expect that.
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08-10-2012, 11:38 PM
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#3 |
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Senior Member
Location: San Francisco, CA Join Date: Feb 2011
Posts: 286
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Could be Poly-A tails too, if this is RNA-seq, or if you just didn't RNase your samples that well. Transcriptome assemblies have more absurd poly-A coverage than that though.
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08-11-2012, 09:07 AM
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#4 |
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Super Moderator
Location: US Join Date: Nov 2009
Posts: 437
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Looks like polyA or lots of Alu sequences. What is the source of the data. This would help us debug.
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