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Old 03-19-2013, 01:26 PM   #1
hspitia
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Location: Colombia

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Default Full-length cDNA and NGS

Hello everyone,

Is there such a thing such as full-length cDNA libraries that can be sequenced through NGS?

Thanks,

Héctor
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Old 03-19-2013, 01:54 PM   #2
kwaraska
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If by full length you mean sequence each base-yes. Shear it to appropriate size and make a library. There will be enouch overlap to reconstruct the full length.

If by full length you mean in one continuous sequence-no. Even Sanger does not go that long.
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Old 03-20-2013, 10:11 AM   #3
madbomber
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Not sure if this answers your question but here is a publication and poster showing full length cDNA sequencing to look at splice isoforms without assembly:
http://www.pacificbiosciences.com/pd...g_PacBioRS.pdf

http://www.plosone.org/article/info%...l.pone.0046679
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