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Does small RNA sequencing generate comparable reads as regular Solexa sequencing? Goodluck Illumina/Solexa 1 08-19-2009 02:12 AM

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Old 05-16-2014, 05:08 AM   #1
Schelarina
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Default small RNA sequencing

Hello,
TruesmallRNASeq kit is able to clone and sequence both strands of the small RNA?
Thanks

Last edited by Schelarina; 05-16-2014 at 05:13 AM.
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Old 05-16-2014, 09:38 AM   #2
kmcarr
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Small RNAs, like the majority of RNA species are single stranded, unless I am misinterpreting your meaning of "both strands".
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Old 05-16-2014, 02:08 PM   #3
Schelarina
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small RNAs are first processed as double stranded RNA and then dissociate and the sense strand is ligation competent. I am not 100% if the antisense strand which has P group at 3'end can also be ligated, that s why I ve asked..
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Old 05-16-2014, 04:20 PM   #4
nucacidhunter
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Ligation reaction occurs between 3' OH of one nucleic acid strand with 5' phosphate of another strand. 3' P group would inhibit ligation and amplification.
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