Go Back   SEQanswers > Literature Watch

Similar Threads
Thread Thread Starter Forum Replies Last Post
PubMed: Next generation massively parallel sequencing of targeted exomes to identify Newsbot! Literature Watch 0 01-29-2011 11:50 AM
PubMed: Deep sequencing to reveal new variants in pooled DNA samples. Newsbot! Literature Watch 0 10-21-2009 03:02 AM
PubMed: SNP frequency estimation using massively parallel sequencing of pooled DNA. Newsbot! Literature Watch 0 10-16-2008 06:00 AM
PubMed: Keeping Up With the Next Generation. Massively Parallel Sequencing in Clinica Newsbot! Literature Watch 0 10-04-2008 06:02 AM
PubMed: Massively parallel synthetic sequencing of DNA on clonal arrays Newsbot! Literature Watch 0 02-21-2008 03:06 PM

Thread Tools
Old 05-12-2010, 03:01 AM   #1
RSS Posting Maniac

Join Date: Feb 2008
Posts: 1,443
Default PubMed: Massively Parallel Sequencing of Pooled DNA Samples--The Next Generation of M

Syndicated from PubMed RSS Feeds

Massively Parallel Sequencing of Pooled DNA Samples--The Next Generation of Molecular Markers.

Genetics. 2010 May 10;

Authors: Futschik A, Schlötterer C

Next generation sequencing (NGS) is about to revolutionize genetic analysis. Currently NGS techniques are mainly used to sequence individual genomes. Due to the high sequence coverage required, the costs for population scale analyses are still too high to allow an extension to non-model organisms. Here, we show that NGS of pools of individuals is often more effective in SNP discovery and provides more accurate allele frequency estimates, even when taking sequencing errors into account. We modify the population genetic estimators Tajima's pi and Watterson's to obtain unbiased estimates from NGS pooling data. Given the same sequencing effort, the resulting estimators often show a better performance than those obtained from individual sequencing. Although our analysis also shows that NGS of pools of individuals will not be preferable under all circumstances, it provides a cost effective approach to estimate allele frequencies on a genome-wide scale.

PMID: 20457880 [PubMed - as supplied by publisher]

Newsbot! is offline   Reply With Quote

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 05:21 PM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2021, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO