Can I use the accepted_hits.bam of "tophat2 --fusion-search" to calculate the gene expression level?
Usually I just use tophat to map reads to genome, then calculate the gene expression level by the accepted_hits.bam. But when I need to rsearch the fusion transcript, I use the "tophat2 --fusion-search", then I got an accepted_hits.bam, could I use this bam file to calculate the gene expression level? I don't want to map the reads again using tophat2 without --fusion-search.
Thanks!
Usually I just use tophat to map reads to genome, then calculate the gene expression level by the accepted_hits.bam. But when I need to rsearch the fusion transcript, I use the "tophat2 --fusion-search", then I got an accepted_hits.bam, could I use this bam file to calculate the gene expression level? I don't want to map the reads again using tophat2 without --fusion-search.
Thanks!