Recently, i faced with a problem, I want to overlap the sequences in order to have a longer one, but i can't find out a assembler which could be suitable to my data.
The length of my data is wide range, from 102bp to 87249bp, with N50 length of 738bp, so it count not be assembled using 454 data assembler, such as Newbler.
What's more, the total bases number of my data is about 1.9G, so it seems to be impossible to use Sanger data assembler.
What i want to do is to extend the sequences based on the overlap relationship between them, if someone have any suggestion, please inform me.
thank you
The length of my data is wide range, from 102bp to 87249bp, with N50 length of 738bp, so it count not be assembled using 454 data assembler, such as Newbler.
What's more, the total bases number of my data is about 1.9G, so it seems to be impossible to use Sanger data assembler.
What i want to do is to extend the sequences based on the overlap relationship between them, if someone have any suggestion, please inform me.
thank you
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