Go Back   SEQanswers > Bioinformatics > Bioinformatics

Similar Threads
Thread Thread Starter Forum Replies Last Post
Interpretation of Picard's MEDIAN_CV_COVERAGE Lien Bioinformatics 3 04-28-2015 02:50 AM
Interpretation of bwa output - has it used all the reads gwilymh Bioinformatics 0 10-16-2014 09:38 AM
Quick Interpretation of FastQC output rnastar Bioinformatics 4 11-22-2013 12:43 PM
interpretation of output from HTSeq syintel87 Bioinformatics 1 01-06-2013 02:12 PM
Varscan-output interpretation bioman1 Bioinformatics 0 05-24-2012 01:50 PM

Thread Tools
Old 04-25-2019, 07:40 AM   #1
Junior Member
Location: UK

Join Date: Feb 2012
Posts: 8
Default Picard InsertSizeMetrics Output Interpretation


I am looking at the data from my pilot Miseq Nano run performed on ATAC-seq libraries.
I wanted to see whether the fragment size distribution pattern corresponds to the one normally observed in ATAC libraries (nucleosome-free regions followed by nucleosomes), and ran Picard InsertSizeMetrics. I'm struggling with the interpretation of its output: what's he difference between FR count and RF count? Why am I getting distributions that are so different? Please see the plots attached. Thanks!
Attached Images
File Type: png Insert size FR title.png (41.4 KB, 6 views)
File Type: png Insert size RF title.png (35.4 KB, 5 views)
OlgaMikh is offline   Reply With Quote

atac-seq, illumina, insertsize, picard

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 12:03 AM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2021, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO