Tweet
No problem, good luck with your further analysis.
-
-
bowtie-build error
Hi boetsie,Originally posted by boetsie View Post
SSPACE must be very useful tool for scaffolding. But when I tried to use it, the process was failed by bowtie-build step. I only have contig file contains all name with super_contig sequences without other information and there are lots of 'N' gaps between. Do I need to modify some information and get bowtie-build works? If not, what's the problem?
The reads I have are 100PE
so the library is like
lib1 ***1.fastq ***2.fastq 200 0.7 0
or I should replace 200 to 400?Comment
-
The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...04-22-2024, 07:01 AM -
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM
Comment