Hi all,
Have two experiments, one run on HiSeq PE125 and the other on NextSeq PE75, same V4 chemistry for both. I have thus far chopped 49bp off the PE125 output to get the same 76bp data as from NextSeq to combine replicates. It seems to be ok in terms of downstream peak calling etc, wondering everyones thoughts on whether this is kosher and if so is there a best approach? Thanks!
Have two experiments, one run on HiSeq PE125 and the other on NextSeq PE75, same V4 chemistry for both. I have thus far chopped 49bp off the PE125 output to get the same 76bp data as from NextSeq to combine replicates. It seems to be ok in terms of downstream peak calling etc, wondering everyones thoughts on whether this is kosher and if so is there a best approach? Thanks!
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