SEQanswers

Go Back   SEQanswers > Sequencing Technologies/Companies > Ion Torrent



Similar Threads
Thread Thread Starter Forum Replies Last Post
comparing exome sequencing and exome chip data Rabu Bioinformatics 0 10-28-2014 11:40 AM
PCR duplicate removal for whole genome sequencing vs. whole exome sequencing cliff Bioinformatics 1 09-27-2011 08:29 AM
Hands-on ngs workshop - human exome sequencing and microbial whole genome sequencing vikram Events / Conferences 0 12-08-2010 09:36 PM

Reply
 
Thread Tools
Old 08-18-2015, 11:10 PM   #1
Shiwali Goyal
Junior Member
 
Location: India

Join Date: Aug 2015
Posts: 9
Default Exome sequencing troubleshootings

Im doing whole exome sequencing on Ion torrent platform. I started with 1.4microgram/microliter of stock DNA. The fragmentation of the DNA was done correctly as I can see the smear on E-gel near 130-150basepair. Next I proceed with the adapter ligation of the library and then to the size selection step of the library preparation. On library preparation step I picked up the library of 210 basepair size. Then I proceed with the size selected library amplification. When I checked the Qubit concentration of my amplified library, it was below 4 ng/microliter (required 500ng). This concentration must be near 10 or 11 ng/microlitre. Can anyone tell me where the flaw is in this whole experiment?
Shiwali Goyal is offline   Reply With Quote
Reply

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 10:39 PM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2018, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO