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I would like to sequence PCR amplicons with Ilumina. The PCR amplicon are between 200bp to 450bp. These are PCR amplicons from one gene already pooled for one patient.I will index each patient. I have heard that the PCR amplicons might be to small to use with the sample prep kit from Ilumina? Also for sequencing there might be a to big difference between the amplicons to sequence them together. Anyone with experience in ampliconsequencing that can give me some good advice?
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...04-22-2024, 07:01 AM -
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM