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Old 12-21-2010, 07:21 AM   #1
manisisland
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Default Sure Select Exon Capture

Hey Guys,

For Agilent Sure Select All Exon kit, what is your experience with hybridization? The protocol suggests 24 to 72 hr. What is the standard practice now?

Thanks,
Manisisland
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Old 12-21-2010, 12:12 PM   #2
ntremblay
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We have always used 24 hrs hybridization and always obtain great results ... also minimizing the hybridization time reduce the evaporation risk and the total hands-on time required.

The only time I've used the 72 hrs is when I've prepared libraries on a friday and left them for the week-end!
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Old 12-21-2010, 02:21 PM   #3
manisisland
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Thanks ntremblay!
By the way, what is your on target rate?
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Old 12-21-2010, 03:59 PM   #4
ntremblay
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Our on target rate is about 75% within 100 bp ...
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