RT Enzyme for template switching in SMART cDNA

[snotarba]

Dears,

does anyone know what is the best commercially available RT (M-MULV) enzyme to add C bases at the end of cDNA for template switching during SMART cDNA sysntesis?

Thanks a lot!

Samuele

[bagnall.lab]

check the single cell thread. There has been a lot of discussion recently as new lots SSII seems to be giving some labs a level of bacterial contamination.