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  • Sample titrations

    i Everyone,

    I'd just like to get an idea of how many (if any) sample titrations
    people are using for cluster generation when the sample has never been
    sequenced before (although the G+C content is known) and the genome is
    small (i.e. bacterial or viral genomes, megaplasmids etc). What about
    DGE samples? Do people still follow the recommendation in the Illumina
    documentation?

    Cheers.

  • #2
    I perform mainly ChiP-Seq and don't titrate samples at all. It did it once shortly after the GA was installed sticking since then to a 2,5pM concentration (measured with Agilents bioanalyzer) yielding 22-32k colonies / tile (GA1!).

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