Hi folks,
I wonder if someone can help with a few beginner questions around poly(A) and RNA-seq?
We used the standard Illumina kit to purify poly(A) RNA for rna-seq. I'm trying to understand the repercussions of this for the various types of RNA.
mRNA
Most mRNA will get through to the reading stage. Is this correct?
rRNA
I've been told that some will get through. Is this because some rRNA is poly(A), or because some non-poly(A) RNA gets through.
ncRNA
Do non-coding transcripts tend to have poly(A) tails? I've getting mixed messages about this - perhaps it is an area that is not fully understood?
tRNA
Any ideas?
Thanks for your time!
I wonder if someone can help with a few beginner questions around poly(A) and RNA-seq?
We used the standard Illumina kit to purify poly(A) RNA for rna-seq. I'm trying to understand the repercussions of this for the various types of RNA.
mRNA
Most mRNA will get through to the reading stage. Is this correct?
rRNA
I've been told that some will get through. Is this because some rRNA is poly(A), or because some non-poly(A) RNA gets through.
ncRNA
Do non-coding transcripts tend to have poly(A) tails? I've getting mixed messages about this - perhaps it is an area that is not fully understood?
tRNA
Any ideas?
Thanks for your time!
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