Just wanted to say "Hello" to everyone, and glad to see this wonderful site up and running as I will probably have many questions in the near future. I'm a post doctorate researcher, and have some experience with 454 sequencing of bacterial genomes. However, the genomes that I have experience were fairly small (~1.8 Mb) compared to the genomes that I'm now working with (~5.5 Mb), in addition the new genomes have lots of bacteriophage and inverted repeats. The genomes have been assembled [I]de novo[I] using paired end and whole shotgun sequencing, however there are still over 200 contigs per genome. I'm in the process of filling the gaps using PCR and Sanger sequencing, however any suggestions that people have about ways to assemble would be much appreciated.
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...-
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Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...-
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04-04-2024, 04:25 PM -
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