Greetings
We are attempting to sequence microbial genomes with paired-end 454 data supplemented with Illumina/Solexa GAII data to resolve homopolymers etc. Newbler assembly of the 454 data has generated some very nice scaffolds but I have detected at least one inverted contig. I was wondering if anybody knows a means of viewing the Newbler output that will show the paired end relationships as a means of detecting those that are inconsistent. It seems to me that the ACE file does not include scaffold information and Newbler generated scaffolds are simple multifasta files. Any assistance would be most appreciated.
Cheers and thanks
We are attempting to sequence microbial genomes with paired-end 454 data supplemented with Illumina/Solexa GAII data to resolve homopolymers etc. Newbler assembly of the 454 data has generated some very nice scaffolds but I have detected at least one inverted contig. I was wondering if anybody knows a means of viewing the Newbler output that will show the paired end relationships as a means of detecting those that are inconsistent. It seems to me that the ACE file does not include scaffold information and Newbler generated scaffolds are simple multifasta files. Any assistance would be most appreciated.
Cheers and thanks
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