Hi all,
I need to extract DNA from some frustrating marine invertebrates. They are slimy and have a pigment that must have some chemical properties similar to DNA. When I do an extraction with phenol/chloroform, the Qiagen DNeasy kit, or even the MoBio PowerSoil kit, the resulting DNA is dark brown when resuspended in water. Nanodrop can't make heads or tails of it. I'm fairly limited on material (this is a deep sea, Antarctic species) and I need about 50 micrograms of high molecular weight DNA for an Illumina mate pair library. I was wondering if you have any extraction or re-precipitation suggestions. Maybe Ampure beads?
Thanks!
Kevin
I need to extract DNA from some frustrating marine invertebrates. They are slimy and have a pigment that must have some chemical properties similar to DNA. When I do an extraction with phenol/chloroform, the Qiagen DNeasy kit, or even the MoBio PowerSoil kit, the resulting DNA is dark brown when resuspended in water. Nanodrop can't make heads or tails of it. I'm fairly limited on material (this is a deep sea, Antarctic species) and I need about 50 micrograms of high molecular weight DNA for an Illumina mate pair library. I was wondering if you have any extraction or re-precipitation suggestions. Maybe Ampure beads?
Thanks!
Kevin
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