SEQanswers

Go Back   SEQanswers > Applications Forums > Metagenomics



Similar Threads
Thread Thread Starter Forum Replies Last Post
PubMed: Exploring of new Y-chromosome SNP loci using Pyrosequencing and the SNaPshot Newsbot! Literature Watch 0 07-23-2011 07:10 AM
Current status of nanopore and iontorrent fahim The Pipeline 4 05-11-2011 04:08 PM
PubMed: Oral Bacterial Genome Sequencing Using the High-Throughput Roche Genome Seque Newsbot! Literature Watch 0 08-19-2010 07:50 AM
Current status de novo assembly 454 vs Solexa AlexB Bioinformatics 10 03-21-2010 05:06 PM

Reply
 
Thread Tools
Old 07-25-2009, 06:07 AM   #1
Newsbot!
RSS Posting Maniac
 

Join Date: Feb 2008
Posts: 1,443
Default PubMed: Exploring the oral bacterial flora: current status and future directions.

Syndicated from PubMed RSS Feeds

Exploring the oral bacterial flora: current status and future directions.

Oral Dis. 2009 Jul 16;

Authors: Parahitiyawa NB, Scully C, Leung WK, Yam WC, Jin LJ, Samaranayake LP

Oral Diseases (2009) doi: 10.1111/j.1601-0825.2009.01607.xObjective: The oral cavity forms an indispensable part of the human microbiome, for its unique and diverse microflora distributed within various niches. While majority of these organisms exhibit commensalism, shifts in bacterial community dynamics cause pathological changes within oral cavity and distant sites. The aim of this review was to appraise the current and emerging methods of detecting bacteria of the oral cavity paying particular attention to the cultivation independent methods. Design: Literature pertaining to cultivation based and cultivation independent methods of oral bacterial identification was reviewed. Methods: The specific advantages and disadvantages of cultivation based, microscopic, immunological and metagenomic identification methods were appraised. Results: Because of their fastidious and exacting growth requirements, cultivation based studies grossly underestimate the extent of bacterial diversity in these polymicrobial infections. Culture independent methods deemed more sensitive in identifying difficult to culture and novel bacterial species. Conclusion: Apart from characterizing potentially novel bacterial species, the nucleic acid sequence data analyzed using various bioinformatics protocols have revealed that there are in excess of 700 bacterial species inhabiting the mouth. Moreover, the latest pyrosequencing based methods have further broadened the extent of bacterial diversity in oral niches.

PMID: 19627515 [PubMed - as supplied by publisher]



More...
Newsbot! is offline   Reply With Quote
Reply

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 09:45 PM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2021, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO