Go Back   SEQanswers > Literature Watch

Similar Threads
Thread Thread Starter Forum Replies Last Post
RNA-Seq: PrimerBank: a PCR primer database for quantitative gene expression analysis, Newsbot! Literature Watch 0 11-17-2011 04:30 AM
RNA-Seq: The sensitivity of massively parallel sequencing for detecting candidate inf Newsbot! Literature Watch 0 05-24-2011 03:00 AM
RNA-Seq: Ribosomal RNA Depletion for Massively Parallel Bacterial RNA-Sequencing Appl Newsbot! Literature Watch 0 03-25-2011 03:00 AM
PubMed: Genome-wide allele-specific expression analysis using Massively Parallel Sign Newsbot! Literature Watch 0 02-21-2008 03:06 PM
PubMed: Gene expression profiling by massively parallel sequencing Newsbot! Literature Watch 0 02-21-2008 03:06 PM

Thread Tools
Old 04-02-2009, 06:00 AM   #1
RSS Posting Maniac

Join Date: Feb 2008
Posts: 1,443
Default PubMed: RNA-Seq - Quantitative measurement of expression through massively parallel R

Syndicated from PubMed RSS Feeds

Related Articles RNA-Seq - Quantitative measurement of expression through massively parallel RNA-sequencing.

Methods. 2009 Mar 28;

Authors: Wilhelm BT, Landry JR

The ability to quantitatively survey the global behavior of transcriptomes has been a key milestone in the field of systems biology, enabled by the advent of DNA microarrays. While this approach has literally transformed our vision and approach to cellular physiology, microarray technology has always been limited by the requirement to decide, a priori, what regions of the genome to examine. While very high density tiling arrays have reduced this limitation for simpler organisms, it remains an obstacle for larger, more complex, eukaryotic genomes. The recent development of "next-generation" massively parallel sequencing (MPS) technologies by companies such as Roche (454 GS FLX), Illumina (Genome Analyzer II), and ABI (AB SOLiD) has completely transformed the way in which quantitative transcriptomics can be done. These new technologies have reduced both the cost-per-reaction and time required by orders of magnitude, making the use of sequencing a cost-effective option for many experimental approaches. One such method that has recently been developed uses MPS technology to directly survey the RNA content of cells, without requiring any of the traditional cloning associated with EST sequencing. This approach, called "RNA-seq", can generate quantitative expression scores that are comparable to microarrays, with the added benefit that the entire transcriptome is surveyed without the requirement of a priori knowledge of transcribed regions. The important advantage of this technique is that not only can quantitative expression measures be made, but transcript structures including alternatively spliced transcript isoforms, can also be identified. This article discusses the experimental approach for both sample preparation and data analysis for the technique of RNA-seq.

PMID: 19336255 [PubMed - as supplied by publisher]

Newsbot! is offline   Reply With Quote

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 12:53 AM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2021, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO