SEQanswers

Go Back   SEQanswers > Introductions



Similar Threads
Thread Thread Starter Forum Replies Last Post
Bowtie beginner question... milesgr General 6 03-14-2012 07:21 AM
A real beginner's question on metagenomics! Plz help! metaStudent General 5 10-24-2011 03:33 AM
Beginner dr.m.umer Epigenetics 1 09-20-2011 04:14 AM
Advice for beginner govanjarz Bioinformatics 0 06-27-2011 04:53 PM
A beginner gongzhichang Introductions 0 06-03-2011 01:55 AM

Reply
 
Thread Tools
Old 03-07-2012, 03:25 AM   #1
mailtovishnupad
Junior Member
 
Location: India

Join Date: Mar 2012
Posts: 1
Smile Hi Iam a beginner to metagenomics kindly help,

iam starting my Phd work in marine metagenomics, I need a basic guidance about this topic. I neither have bio technical nor microbiological background so unable to get a grip on the DNA extraction, cloning, PCR and sequencing. Even a pinch of data is sincerely appreciated. My understanding about the topic is metagenomics is used as an culture independent method to understand the environmental microbes.
As my knowledge on this topic is very limited i am unable to understand and acknowledge the technical advancement and appropriate use of these techniques to implement. My questions are
1.What is the pipeline to proceed from sample collection till sequencing.
2.Some articles speaks on DNA extraction then cloning and library construction but some gives other insights.
3.What should i do to get the sequence from a marine sample.
Awaiting for the replies.

regards
Vishnu

Last edited by mailtovishnupad; 03-07-2012 at 03:27 AM.
mailtovishnupad is offline   Reply With Quote
Reply

Tags
metagenomics, pipeline development, protocol, sequencing

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 12:04 PM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2021, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO