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Old 03-07-2012, 03:25 AM   #1
Junior Member
Location: India

Join Date: Mar 2012
Posts: 1
Smile Hi Iam a beginner to metagenomics kindly help,

iam starting my Phd work in marine metagenomics, I need a basic guidance about this topic. I neither have bio technical nor microbiological background so unable to get a grip on the DNA extraction, cloning, PCR and sequencing. Even a pinch of data is sincerely appreciated. My understanding about the topic is metagenomics is used as an culture independent method to understand the environmental microbes.
As my knowledge on this topic is very limited i am unable to understand and acknowledge the technical advancement and appropriate use of these techniques to implement. My questions are
1.What is the pipeline to proceed from sample collection till sequencing.
2.Some articles speaks on DNA extraction then cloning and library construction but some gives other insights.
3.What should i do to get the sequence from a marine sample.
Awaiting for the replies.


Last edited by mailtovishnupad; 03-07-2012 at 03:27 AM.
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metagenomics, pipeline development, protocol, sequencing

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