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Old 03-17-2012, 09:15 AM   #1
smadbro
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Default I'm a beginner! Help?

Hi guys, I'm a beginner I'm trying to understand next gen sequencing. Basically I'm writing a project up in which I extracted DNA/RNA for next generation sequencing. I'm writing at why I would require high molecular weight DNA/RNA in order to construct libraries. I don't really understand what libraries this means? I think it could mean the sheared fragments that are clonally amplified before using NGS on them, but I'm very stuck. Are there any papers you could link me to which would make this sort of thing clearer?

Thanks alot!
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Old 03-18-2012, 05:19 PM   #2
HESmith
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Most protocols explain the rationale for using high MW material; that would be a good place to start.
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Old 11-20-2012, 07:01 PM   #3
jeev
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What would the explanation be? I've not seen a direct reason the protocols suggesting it so far.
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Old 11-20-2012, 07:25 PM   #4
marcowanger
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Quote:
Originally Posted by smadbro View Post
Hi guys, I'm a beginner I'm trying to understand next gen sequencing. Basically I'm writing a project up in which I extracted DNA/RNA for next generation sequencing. I'm writing at why I would require high molecular weight DNA/RNA in order to construct libraries. I don't really understand what libraries this means? I think it could mean the sheared fragments that are clonally amplified before using NGS on them, but I'm very stuck. Are there any papers you could link me to which would make this sort of thing clearer?

Thanks alot!
For general idea: http://en.wikibooks.org/wiki/Next_Ge...quencing_(NGS)
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