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Old 08-01-2014, 05:36 PM   #1
sindrle
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Default Gene expression and lipids, time-series analysis

Hi!
I have two data sets; genes and lipids (RNAseq and lipidomics).
I have 6 time points (equal for both data sets).

How can I see how these two data sets influence each other? Should I use i.e. "MixOmics" - approaches, or are there some time-series correlation methods I could use?

Or simply, how do I know if the time-serie of gene X correlate with the time-serie of lipid Y?

Thank you!
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Old 08-01-2014, 09:52 PM   #2
aby
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What organism are you using ? What pathway ?
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Old 08-02-2014, 04:08 AM   #3
sindrle
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Human. Insulin pathway and lipid metabolism are of great interest!
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Old 08-02-2014, 04:52 AM   #4
sindrle
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For simplicity, say you measured 5 gener using RT-PCR, and you measured 8 lipid species. All within the same tissue and at the same 6 time points. And you have 26 observations in each time point for each gene and lipid.

How can you decide the correlation between these series?

Thank you!

Last edited by sindrle; 08-02-2014 at 06:05 AM.
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Old 08-02-2014, 08:14 AM   #5
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You might want to use R time series ARIMAX modeling to see time series relation between two series and then model.
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Old 08-02-2014, 08:24 AM   #6
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Ok, thank you, Ill try that.
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