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Sanger sequencing Sunita Sanger/Dye Terminator 1 08-23-2017 11:02 AM

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Old 04-16-2018, 12:32 PM   #1
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Location: Athens, Ohio

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Default Sanger Sequencing for Community Analysis

I have environmental cDNA left over from a tricky qPCR project. Is it possible to use Sanger Sequencing of the ITS 5.8S rDNA region to observe the community at even the order level?
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Old 04-17-2018, 05:32 PM   #2
David Eccles (gringer)
Location: Wellington, New Zealand

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Unlikely. Sanger sequencing doesn't really work with variants at a frequency below about 20%, and any INDEL variation in the community is going to mess up the sequencing quite a lot.
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5.8s, community analysis, fungi, sanger

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