Hi, I've been lurking these forums for a while, and this is my first time posting.
I'm running an RNA-Seq experiment that will use 4 lanes and will be multiplexing all of my samples on each lane. The sequencing facility that I will be sending samples to has two Illumina HiSeq machines, one with 8 lanes, and another that has 2 lanes. Would there be a difference between sequencing my samples on the 8 lane machine (all 4 lanes run on same batch), or sequencing my samples on the 2 lane machine (run 2 lanes, then run the "third and fourth" lanes on another sequencing run)?
Thanks!
I'm running an RNA-Seq experiment that will use 4 lanes and will be multiplexing all of my samples on each lane. The sequencing facility that I will be sending samples to has two Illumina HiSeq machines, one with 8 lanes, and another that has 2 lanes. Would there be a difference between sequencing my samples on the 8 lane machine (all 4 lanes run on same batch), or sequencing my samples on the 2 lane machine (run 2 lanes, then run the "third and fourth" lanes on another sequencing run)?
Thanks!
Comment