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Old 09-08-2013, 02:20 PM   #1
arkilis
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Default What is the diff among Illumina Genome Analyzer, Miseq and hiseq?

Asin the title. Stupid question... But hope someone can help, thx
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Old 09-08-2013, 02:53 PM   #2
scbaker
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Originally Posted by arkilis View Post
Asin the title. Stupid question... But hope someone can help, thx
They all use basically the same chemistry (sequencing-by-synthesis), but there are many differences in terms of the mechanics and optics of the various instruments. More importantly for the end user are the differences in cost, throughput and minimum run time. Have a look at our entry on Illumina in BlueSEQ's NGS Knowledge Bank to get a better idea: http://blueseq.com/knowledgebank/seq...orms/illumina/
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Old 09-08-2013, 04:12 PM   #3
arkilis
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Originally Posted by scbaker View Post
They all use basically the same chemistry (sequencing-by-synthesis), but there are many differences in terms of the mechanics and optics of the various instruments. More importantly for the end user are the differences in cost, throughput and minimum run time. Have a look at our entry on Illumina in BlueSEQ's NGS Knowledge Bank to get a better idea: http://blueseq.com/knowledgebank/seq...orms/illumina/
Thanks. As far as I see the three (Genome sequencer, miseq and hiseq) are quite similar. But diff on the run output.
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Old 09-08-2013, 05:16 PM   #4
scbaker
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They are somewhat similar, but here are the basic differences:

Genome Analyzer - original machine; still being sold, but no longer being updated; probably not the best choice
HiSeq - main workhorse, most expensive, highest output; best choice if you have a large number of samples or need a lot of reads per sample
MiSeq - the 'new' desktop instrument with quick and inexpensive runs; best choice if you have a smaller number of samples and if you need quick turnaround times.

Libraries for the HiSeq and MiSeq are compatible, so many HiSeq owners also have a MiSeq or two for testing purposes and other small projects.

These differences are really only important if you're looking to buy your own instrument. If you're outsourcing it doesn't really matter - just pick the best combination of cost and turnaround time that fits your needs.
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Old 09-08-2013, 05:24 PM   #5
arkilis
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Originally Posted by scbaker View Post
They are somewhat similar, but here are the basic differences:

Genome Analyzer - original machine; still being sold, but no longer being updated; probably not the best choice
HiSeq - main workhorse, most expensive, highest output; best choice if you have a large number of samples or need a lot of reads per sample
MiSeq - the 'new' desktop instrument with quick and inexpensive runs; best choice if you have a smaller number of samples and if you need quick turnaround times.

Libraries for the HiSeq and MiSeq are compatible, so many HiSeq owners also have a MiSeq or two for testing purposes and other small projects.

These differences are really only important if you're looking to buy your own instrument. If you're outsourcing it doesn't really matter - just pick the best combination of cost and turnaround time that fits your needs.
Thanks!! you are really helpful!
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