Hello all,
I was wondering what is the best protocol to sequence several bacterial genomes (1-2Mb in size) using the newest flowcells (R9) and and a MinION sequencer.
We want to assemble them de novo; if need be, we can add some Illumina reads as well.
It seems like ligation protocol 1D should give the best yield. I am struggling in finding the exact protocol with times etc. If you could point me to protocols that work best on the newest flowcells I'd be most grateful.
I was also wondering what is a good strategy for sequencing several genomes in 1 run - since it seems an overkill to run only one per flowcell. I know you can turn it off, but are there any good pooling strategies? Just adding things as we go on sequencing?
I was wondering what is the best protocol to sequence several bacterial genomes (1-2Mb in size) using the newest flowcells (R9) and and a MinION sequencer.
We want to assemble them de novo; if need be, we can add some Illumina reads as well.
It seems like ligation protocol 1D should give the best yield. I am struggling in finding the exact protocol with times etc. If you could point me to protocols that work best on the newest flowcells I'd be most grateful.
I was also wondering what is a good strategy for sequencing several genomes in 1 run - since it seems an overkill to run only one per flowcell. I know you can turn it off, but are there any good pooling strategies? Just adding things as we go on sequencing?
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